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24 Under normal circumstances, to preserve high inuacellular concentrations of free GSH, GSSG is reduced rapidly back to GSH by the NADPH-dependent GSSG reductase

Although the direct cytotoxic effects of high cellular GSSG levels have been documented in a variety of cell types 33,34,35,36 , our cell culture studies with hepatocytes revealed that increased GSSG accumulation, despite being non-toxic per se, could sensitize hepatocytes to TNF-induced killing, suggesting that GSSG and TNF can synergistically induce hepatocyte death and that GSSG-triggered sensitization to TNF hepatotoxicity represents a clinically relevant process that may account for disease progression in NAFLD

doi: 10.3389/fendo.2019.00510 169 SabatinoL

Standard protocol: let the BPC vial and your bacteriostatic water come to room temperature, wipe both stoppers with a 70% isopropyl swab, then inject the water slowly down the side wall of the peptide vial